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NAD Side Effects: What Injection, IV and Oral Precursor Studies Reported

IV drips caused stomach symptoms and chest pressure during the infusion. Shots under the skin have never been studied. Oral building blocks have randomized trials with clean short-term records.

Article type
Safety
Evidence on NAD+ for longevity
Early human dataOnly uncontrolled or dosing studies in people are cited here for longevity (2 studies), with no comparison group.
Sources
7
Reading
6 min
By Leah GarnerPublished

NAD side effects depend on the route, and the three routes people use have very different records. Drips have one study of what people felt. Injections under the skin have none. Pills made from its building blocks have several randomized trials.

This page keeps them apart, because a finding for one route says little about the others. What the drips were tested for is on NAD IV therapy, and the claims made for injections on NAD+ injections. NAD+ sits under the longevity goal here.

What has been recorded about side effects, by route
RouteHuman safety dataWhat it recorded
IV drip of NAD+One records review from a commercial clinicStomach symptoms, faster heartbeat and chest pressure during the drip
Injection under the skinNone publishedNot studied in people
Nasal sprayNone publishedStudied only in mice and cells
Oral nicotinamide ribosideRandomized trials, 4 to 12 weeksNo more side effects than placebo
Oral NMNA 2026 meta-analysis of randomized trialsNo rise in side effects or liver enzymes
What has been recorded about side effects, by route The studies cited on this page.

IV drips: symptoms during the infusion

The clearest side-effect record for NAD+ itself comes from a 2026 review of clinic records [1]. Clients received 500 mg of NAD+ by vein on four days in a row. A comparison group received the same dose of nicotinamide riboside, a building block of NAD+.

The NAD+ group reported moderate to severe stomach symptoms, a faster heartbeat and chest pressure during the infusions. All of it resolved when the drip ended. The symptoms made NAD+ drips last 97 minutes on average, against 37 minutes for the comparison drip.

Blood tests of the liver, kidneys, thyroid and inflammation did not change over 30 days. One enzyme, alkaline phosphatase, fell in the NAD+ group but stayed in the normal range. HDL cholesterol also fell in that group. The review was retrospective, with no placebo group.

Injections under the skin: not studied

Online sellers commonly prescribe NAD+ as a shot under the skin. No published study has recorded what those shots do in people, good or bad. A 2026 systematic review of NAD+ studies through October 2025 found no outcome trial of NAD+ given by vein or by injection into muscle for aging or wellness [3].

That gap is not proof the shots are safe or unsafe. It means their side effects have not been counted in a study. The nasal form has even less: its only study, covered in NAD nasal spray, was in mice and in human cells in a dish.

Oral building blocks: the best-studied route

Nicotinamide riboside and NMN are not NAD+. They are smaller molecules the body turns into NAD+, sold as supplements. Unlike NAD+ shots, they have been tested in randomized trials with placebo groups.

In an 8-week trial in overweight adults, nicotinamide riboside at 100, 300 or 1,000 mg a day raised blood NAD+ by 22%, 51% and 142% [4]. There were no reports of flushing, and side effects did not differ from placebo at any dose.

A 12-week trial gave 2,000 mg a day to 40 obese men, or placebo [5]. No serious side effects were attributed to it, and safety blood tests were normal. It also found no gain in insulin sensitivity.

The highest dose tested was 3,000 mg a day, split in two, for 4 weeks in 20 people with Parkinson’s disease (n=10 per arm) [6]. There were no moderate or severe side effects. Homocysteine, a blood marker linked to heart risk, rose slightly at first.

For NMN, a 2026 meta-analysis pooled randomized trials at 250 to 2,000 mg a day, lasting 14 days to 24 weeks [7]. NMN did not raise overall, serious or system-specific side effects, or the liver enzymes ALT and AST.

What no study has measured

Every study above ran for weeks or months, the longest about 24 weeks. Nobody has measured what years of NAD+ drips, shots or supplements do. The oral trials also used pills, so their clean safety record does not transfer to a drip or an injection.

If you are weighing a route, the side-effect record is strongest for pills and absent for shots. For how often drips are given and what they cost in time, see NAD IV therapy again, and for a separate compound often sold beside NAD+, see glutathione injections.

Frequently asked questions

What are the side effects of an NAD IV drip?
In a 2026 review of clinic records, people given 500 mg of NAD+ by vein reported moderate to severe stomach symptoms, a faster heartbeat and chest pressure during the infusion. The symptoms resolved when the drip ended, and the drips averaged 97 minutes.
What are the side effects of NAD injections?
They have not been studied. No published study has recorded the effects of NAD+ injected under the skin in people, and a 2026 systematic review found no outcome trial of injected NAD+ for aging or wellness.
Is oral NAD safe?
The oral building blocks of NAD+ have the best short-term record. Nicotinamide riboside up to 1,000 mg a day for 8 weeks caused no more side effects than placebo, and a 2026 meta-analysis found no rise in side effects with NMN at 250 to 2,000 mg a day for up to 24 weeks.
Does NAD cause flushing?
In an 8-week trial of oral nicotinamide riboside at up to 1,000 mg a day, there were no reports of flushing. No published study has measured flushing after NAD+ shots under the skin.

Sources

7 cited
  1. 1Reyna K, Heinzen G, Patel N, et al. (2026). Intravenous infusion of nicotinamide adenine dinucleotide (NAD(+)) versus nicotinamide riboside (NR): a retrospective tolerability pilot study in a real-world setting Frontiers in Aging. PMID 41704678
  2. 2Grant R, Berg J, Mestayer R, et al. (2019). A Pilot Study Investigating Changes in the Human Plasma and Urine NAD+ Metabolome During a 6 Hour Intravenous Infusion of NAD Frontiers in Aging Neuroscience. PMID 31572171
  3. 3Gallagher C, Emmanuel OO (2026). NAD⁺ supplementation for anti-aging and wellness: A PRISMA-guided systematic review of preclinical and clinical evidence Ageing Research Reviews. PMID 41655607
  4. 4Conze D, Brenner C, Kruger CL (2019). Safety and Metabolism of Long-term Administration of NIAGEN (Nicotinamide Riboside Chloride) in a Randomized, Double-Blind, Placebo-controlled Clinical Trial of Healthy Overweight Adults Scientific Reports. PMID 31278280
  5. 5Dollerup OL, Christensen B, Svart M, et al. (2018). A randomized placebo-controlled clinical trial of nicotinamide riboside in obese men: safety, insulin-sensitivity, and lipid-mobilizing effects American Journal of Clinical Nutrition. PMID 29992272
  6. 6Berven H, Kverneng S, Sheard E, et al. (2023). NR-SAFE: a randomized, double-blind safety trial of high dose nicotinamide riboside in Parkinson's disease Nature Communications. PMID 38016950
  7. 7Yang W, Huang J, Tang Z, et al. (2026). Safety and Metabolism-Related Outcomes of Oral Nicotinamide Mononucleotide Supplementation in Adults: A Systematic Review and Meta-Analysis Nutrients. PMID 42514320

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